| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 401 | Caldicellulosiruptor bescii | TTGTCAGGCCAGAGAGGGA | TTCATCCGGTGCTGAGCA | 59.84 | 58.93 | 215 | 46 |
100.00%
|
100.00%
|
| Primer 402 | Caldicellulosiruptor bescii | TTTTCCACCGACCTTGCCC | GCGAAACCTTTGGCAGTGG | 60.53 | 60.01 | 196 | 46 |
100.00%
|
100.00%
|
| Primer 403 | Caldicellulosiruptor bescii | TCGCCAGCCTCAGATGTTC | CGCATTTACTACGGCAGCC | 59.78 | 59.36 | 171 | 46 |
100.00%
|
100.00%
|
| Primer 404 | Caldicellulosiruptor bescii | TCCAAAGGGAAGAACCGGA | TGCAGCTCCTCAAAGGTGT | 58.14 | 59.16 | 234 | 46 |
100.00%
|
100.00%
|
| Primer 405 | Caldicellulosiruptor bescii | TCCAAAGGGAAGAACCGGA | TTCCTGCAGCTCCTCAAAGG | 58.14 | 59.96 | 238 | 46 |
100.00%
|
100.00%
|
| Primer 406 | Caldicellulosiruptor bescii | GCTTCGAGTCAAGCAATGGC | ACCGATTAAAGCTTCAAGGGC | 60.18 | 58.91 | 162 | 46 |
100.00%
|
100.00%
|
| Primer 407 | Caldicellulosiruptor bescii | TCGCGTGATTTGCCTGCA | CGAACAAAGTCCAACTGGCA | 60.67 | 58.98 | 159 | 46 |
100.00%
|
100.00%
|
| Primer 408 | Caldicellulosiruptor bescii | TTCGCGTGATTTGCCTGC | TCGAACAAAGTCCAACTGGCA | 59.44 | 60.41 | 161 | 46 |
100.00%
|
100.00%
|
| Primer 409 | Caldicellulosiruptor bescii | TGCTTGGCAGGAAAGGGGA | TGAACAGTTGGTCTTTGCTGA | 61.47 | 58.00 | 162 | 46 |
100.00%
|
100.00%
|
| Primer 410 | Caldicellulosiruptor bescii | TGGCAGGAAAGGGGAATACA | TGAACAGTTGGTCTTTGCTGA | 58.62 | 58.00 | 158 | 46 |
100.00%
|
100.00%
|